Skip to main content
. 2010 May 21;3:12. doi: 10.3389/fnmol.2010.00012

Figure 8.

Figure 8

Degradation of proteasome components after NMDA exposure. (A) The degradation of 19S subunits. Neuronal lysates collected at 30’ and 4 h post-NMDA were resolved by SDS-PAGE and probed for total levels of 20S subunit α7, 19S subunit Rpt1, and VCP (an abundant UPS protein). Tubulin served as the loading control. (B) Quantification of the results in (A) by densitometry (n = 6, mean ± SEM, *p < 0.05 by paired t-test). (C) Degradation of proteasome-interacting E3s. Neuronal lysates from (A) were immunoblotted against E3s (HUWE1, UBE3A, KCMF1) and DUBs (USP14) that interact with proteasomes. (D) The graph represents protein levels in (C) normalized to α7 levels (n = 5, *p < 0.05 by paired t-test).