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. 2010 May 21;3:12. doi: 10.3389/fnmol.2010.00012

Figure 9.

Figure 9

The degradation of 19S particles is specific to NMDA treatment. (A) Cultured hippocampal neurons were briefly treated with NMDA (20 μM, 3 min) or DHPG (100 μM, 10 min), or persistently treated with TTX (2 μM) or TTX + CNQX + APV (2, 40, and 50 μM, respectively). Only the vehicle was added in control experiments (Con). Neuronal lysates were collected at 4 h after drug addition, and analyzed by SDS-PAGE and Western blot for the levels of 19S subunit Rpt1 and 20S subunit α7. GAPDH served as loading control. (B,C) represent the results in (A) quantified by densitometry. Rpt1 and α7 levels (mean ± SEM) were normalized to the control. Statistical significance (*p < 0.05) was determined by paired t-test against the control (n = 8 for NMDA and DHPG, n = 4 for TTX and TTX + APV + CNQX).