(A) Amplification products of the MCP gene from the 3 Brazilian Rana catesbeiana viral isolates (RCV-BR) (Lanes 1, 2, and 3) and frog virus 3 (Lane 4). Lane 5 is the negative control, and Lane M is the molecular marker (1 kb Plus DNA ladder; Invitrogen), where ‘a’ indicates 1500 bp and ‘b’ 1000 bp. (B) Amplification products of Pol II gene from the 3 RCV-BR isolates (Lanes 1, 2, and 3). Lane 4 is the negative control, and Lane M is the molecular marker (100 kb DNA ladder; Invitrogen), where ‘c’ indicates 500 bp. Purified virus genomic DNA was used as a template in PCR reactions described in ‘Materials and methods’. PCR products were separated on a 1 % agarose gel and visualized by staining with 0.5 μg ml1 ethidium bromide