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. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: Cancer Prev Res (Phila). 2009 May 26;2(6):581–589. doi: 10.1158/1940-6207.CAPR-08-0142

Figure 1. Butyrate down-regulates survivin and induces apoptosis in HT-29/APC cells, but not in HT-29/β-Gal cells.

Figure 1

(A) Butyrate induced apoptosis in HT-29/APC cells but not in HT-29/β-Gal cells. Cells were cultured in media containing 50 μM Zinc for 24h and then treated with various doses of butyrate for 24h. Apoptosis was analyzed by TUNEL assay. (B) Butyrate down-regulated survivin in HT-29/APC cells. HT-29/APC and HT-29/β-Gal cells were cultured in media containing 50 μM ZnCl2 for 24h and then treated with 2 mM butyrate for 24h. APC expression, survivin and β-Tubulin protein levels were detected by western blotting. Relative protein levels were quantified and shown under the gel. The experiments were repeated three times.