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. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: Virology. 2008 May 5;376(2):258–269. doi: 10.1016/j.virol.2007.12.003

Fig. 2.

Fig. 2

Regulation of cell cycle markers in Rat-1 stable cells. Rat-1 cell lines stably expressing LMP1, Id1, or Id3 were analyzed by western blotting. Total cell lysates from control (pBabe), LMP1, myc-tagged Id1 (M3-Id1), or myc-tagged Id3 (M3-Id3) were analyzed for levels of p27, Cdk2 and Rb protein. Actin was used as a loading control and expression of constructs was confirmed by blotting with LMP1-specific and myc-tag antibodies. Fifty micrograms of protein were loaded per lane.