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. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: Virology. 2008 May 5;376(2):258–269. doi: 10.1016/j.virol.2007.12.003

Fig. 5.

Fig. 5

Transient silencing of Id1 in transformation assays. Rat-1 cells were transfected with negative control (panels A, B, E, F) or Id1 siRNA (panels C, D, G, and H) in twelve-well plates. The following day cells were transduced with control HSCG retrovirus (panels A, C, E, G), or LMP1-expressing retrovirus, HSCG-LMP1 (panels B, D, F, H) and focus formation assays were performed for 7 days. Monolayers were fixed, stained with crystal violet, and examined for focus formation by light microscopy using a dissecting microscope. Representative micrographs are depicted at 10X (panels A, B, C, D) or 40X (panels E, F, G, H).