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. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: Virology. 2008 May 5;376(2):258–269. doi: 10.1016/j.virol.2007.12.003

Fig. 9.

Fig. 9

Effects of Id1 silencing on cell growth. Three separate pools of Rat-1 cells were transduced with different retrovirus aliquots expressing control siRNA (open symbols and dashed lines) or Id1 siRNA (closed symbols and solid lines) and selected with puromycin for 48 h. Equal numbers of stable cells from each pool were seeded in duplicate into eight 12-well plates. The next day and each day afterwards, one plate (containing two wells per pool) was fixed and stained with DAPI. Four randomly selected fields for each well were photographed using a fluorescent microscope and DAPI stained nuclei were counted using the ITCN plugin of ImageJ. The average number of cells per field for each pool on each day is graphed.