Figure 3.

Effect of DNRII expression on apoptosis and activation of Erk and Akt in HEC-1-A cells. A) Control and DNRII cells were cultured in six-well plates and harvested at exponential growth phase. Cell Death Detection ELISA was used to measure apoptosis as described in Materials and methods. The values are presented as means ± SEM of three optical density measurements from triplicate wells. B) Exponentially growing cultures of the control (con) and DNRII cells were lysed. Cell lysate were used for measuring the levels of phosphorylated Erk, Akt, and p38, and total PTEN with Western immunoblotting as described in Materials and methods.

Note: *Indicates significant difference from the control value at p < 0.05.