Skip to main content
. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: Anticancer Res. 2009 Aug;29(8):3185–3190.

Figure 2. Curcumin inhibits EGF-stimulated PI3K-Akt-mTOR signaling.

Figure 2

HCT116 cells were serum starved overnight and treated with 25 μM curcumin for 2 hours, followed by stimulation with 20 ng/ml EGF for 45 minutes, prior to cell lysis. Western blotting was performed for rictor, raptor, and the phosphorylated and total forms of mTOR, p70S6K (Thr389), Akt (Ser473) and 4E-BP1 (Thr37/46), with β-Actin serving as a loading control.