Table 3. Effect estimates for alignment from the result of Design Expert analysis.

Coded coefficients are transformed so the high value of the factor becomes +1 and the low value becomes −1 (i.e. for A, 50μm becomes +1 and 10 μm becomes −1).

	Factor	Coefficient (Actual Factor)	Coefficient (Coded Factor)	Standard Error (Coded)	Percent Contribution	P-value
A7	Model					<0.0001*
	Intercept	0.643	0.440	0.009
	A-space width	2.09 × 10−3	−0.031	0.012	14.9%	0.0109*
	B-stripe width	−2.85 × 10−3	−0.057	0.012	47.7%	<0.0001*
	C-plating density	−6.56 × 10−6	−0.015	0.01	4.5%	0.1621
	D-culture time	−1.16 × 10−3	0.012	0.009	4.2%	0.1728
	AD	−1.01 × 10−4	−0.024	0.012	9.0%	0.0468*
	CD	1.57 × 10−7	0.03	0.01	19.6%	0.0037*
	Lack of fit					0.9172**
HUVEC	Model					<0.0001*
	Intercept	0.338	0.290	0.006
	A-space width	−1.34 × 10−3	−0.027	0.009	32.6%	0.0032*
	B-stripe width	1.64 × 10−3	−0.033	0.009	47.3%	0.0005*
	C-plating density	1.13 × 10−6	0.018	0.008	20.1%	0.019*
	Lack of fit					0.7534**
SC	Model					<0.0001*
	Intercept	0.633	0.710	0.005
	A-space width	3.42 × 10−3	−0.013	0.006	11.2%	0.0432*
	C-plating density	1.34 × 10−6	0.022	0.006	42.7%	<0.0002*
	D-culture time	8.22 × 10−4	−0.015	0.005	28.1%	0.0019*
	AD	−6.81 × 10−5	−0.016	0.007	18.0%	0.0140*
	Lack of fit					0.5838**

^*indicates the factor as a significant contributor to the model with p<0.05,

^**indicates an insignificant lack of fit with p>0.05