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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cell Mol Bioeng. 2009 Dec;2(4):554–572. doi: 10.1007/s12195-009-0087-1

Table 5. Numerical optimizations of culture conditions with Design Expert.

Factor Input Bounds Model Output
A7 HUVEC SC
5.1 CONFLUENT
space width 10–50 μm 17–50 μm 10–49 μm 10–50 μm
stripe width 10–50 μm 17–50 μm 10–50 μm 10–50 μm
plating density 17–49K cm −2 45–49K cm−2 29–49K cm−2 17K–49K cm−2
culture time 24–48h
48–72h (SC)		 33–48h 27–48h 48–72h
terminal density 85–125K cm−2 (A7)
32–50K cm−2 (HUVEC)
41–69K cm−2 (SC)		 85–96K cm−2 32–41K cm−2 41–68K cm−2
Desirability 1.000 (60 answers) 1.000 (80 answers) 1.000 (80 answers)
5.2 OPTIMAL ALIGNMENT
space width 10–50μm 10 μm 10 μm 10 μm
stripe width 10–50 μm 10 μm 10 μm 50 μm
plating density 17–49K cm−2 17K cm−2 49K cm−2 49K cm−2
culture time 24–48h
48–72h (SC)		 24h 48h 48h
mean vector length maximize 0.578 ± 0.035 0.373 ± 0.017 0.763 ± 0.014
desirability 0.808 0.752 0.844
5.3 OPTIMAL ALIGNMENT AT CONFLUENCE (IN RANGE OF EXPERIMENTS)
space width 10–50 μm 16 μm 10 μm 10 μm
stripe width 10–50 μm 50 μm 10 μm 49 μm
plating density 17–49K cm−2 49K cm−2 49K cm−2 46K cm−2
culture time 24–48h
48–72h (SC)		 48h 48h 48h
mean vector length maximize 0.498 ± 0.046 0.373 ± 0.017 0.759 ± 0.014
terminal density 85–125K cm−2 (A7)
32–50K cm −2 (HUVEC)
41–69K cm−2 (SC)		 85K ± 8K cm−2 32K ± 2K cm−2 69K ± 6K cm−2
desirability 0.674 0.752 0.829
5.4 MATCHED ALIGNMENT AT CONFLUENCE
space width 10–50 μm 50 μm 10 μm 50 μm
stripe width 10–50 μm 50 μm 10 μm 28 μm
plating density 17–49K cm−2 49K cm−2 49K cm−2 17K cm−2
culture time 24–48h
48–72h (SC)		 31h 48h 72h
mean vector length 0.373
minimize (SC)		 0.376 ± 0.036 0.373 ± 0.017 0.659 ± 0.011
terminal density 85–125K cm−2 (A7)
32–50K cm−2 (HUVEC)
41–69K cm−2 (SC)		 85K ± 6K cm−2 32K ± 2K cm−2 41K ± 3K cm−2
Desirability 1.000 0.752 0.533
5.5 OPTIMAL ALIGNMENT AT CONFLUENCE (LARGER RANGE)
space width 1–100 μm 1–31 μm 1 μm 1–84 μm
stripe width 1–100 μm 1–100 μm 1 μm 1–98 μm
plating density 0–100K cm−2 50–81K cm−2 100K cm−2 45–68K cm−2
culture time 0–72h
0–96h (SC)		 58–72h 18h <1–36h
mean vector length maximize 0.693–0.784 0.435 ± 0.037 0.806–0.863
terminal density 85–125K cm−2 (A7)
32–50K cm−2 (HUVEC)
41–69K cm−2 (SC)		 95–125K cm−2 50K ± 5K cm−2 42–68K cm−2
Desirability 1.000 (75 answers) 0.941 1.000 (77 answers)