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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cell Mol Bioeng. 2009 Dec;2(4):554–572. doi: 10.1007/s12195-009-0087-1

Table 6. Summary of key findings.

A7 HUVEC SC
Most important factor for aligned monolayer: Protein stripe width Cell density Cell density
Other key factors: Protein space width
Cell density
Culture time		 Protein stripe width Culture time
Protein space width
How well optimized: (MVL) 0.498 model
0.443 experimental		 0.373 model*
0.418 experimental* 		0.759 model
0.705 experimental
Suggested mechanism:

  1. Align to stripes.

  2. Fill spaces between stripes by proliferating.

  1. Align to entire stripes.

  2. Fill spaces between stripes by broading to fill spaces.

  1. Align to stripe edges.

  2. Fill spaces between stripes by proliferating and aligning to each other.
*

Note: optimal conditions may be outside range tested.