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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: DNA Repair (Amst). 2009 Jun 18;8(8):886–900. doi: 10.1016/j.dnarep.2009.05.004

FIG. 5.

FIG. 5

The analysis of small deletions at the I-SceI site in EJ-30 clones with interstitial and telomeric integration sites. Small deletions were detected by monitoring the ability of the I-SceI endonuclease to cut a 1.8 kb PCR product spanning the I-SceI site. Genomic DNA was isolated from pooled puror cultures of pQCXIP (A4, B3 and I33) or pQCXIP-ISceI (A4-I, B3-I and I33-I) infected cultures of clones with telomeric integration sites, or pQCXIP (ΔB1, ΔE2 and ΔI27) or pQCXIP-ISceI (ΔB1-I, ΔE2-I and ΔI27-I) infected cultures of clones with interstitial integration sites. The genomic DNA from the pooled puror cells was isolated 10 days after infection with the retroviruses. The percentage of cells that had small deletions was determined by comparing the intensity of the undigested band with the combined intensity of the digested and undigested bands.