FIG. 7.

Southern blot analysis of the types of DNA rearrangements resulting from DSBs near telomeres in Gan^r/G418^r subclones. Genomic DNA from (A) the parental clone A4 and 12 of its Gan^r/G418^r subclones, GGR1 through 12, and (B) the parental clone B3 and 12 of its Gan^r/G418^r subclones infected with pQCXIP-ISceI, GGR1 through 12, was analyzed following digestion with HindIII and hybridization with the pNPTΔ plasmid probe as described in Fig. 4. The data for B3 subclones GGR9–12 were taken from a separate hybridization. Subclones with new highly diffuse bands have undergone chromosome healing, while subclones with new discrete bands have undergone GCRs. (C) The combined percent of cells in clones A4 and B3 with GCRs, chromosome healing (CH), or complete loss (Loss) of the plasmid sequences, when selecting with ganciclovir alone (left), or with ganciclovir and G418 together (right).