Table 1. Summary of the primers used for the amplification of the 17 exons of the human TGFBI gene.
| Exon | Primersequnce(5’→3') | Annealing temperature (°C) | Amplicon length (bp) |
|---|---|---|---|
| 1 |
F: GCGCTCTCACTTCCCTGGAG |
52 |
252 |
| |
R: GACTACCTGACCTTCCGCAG |
|
|
| 2 |
F: GGTGGACGTGCTGATCATCT |
58 |
194 |
| |
R: AGCCAGCGTGCATACAGCTT |
|
|
| 3 |
F: ACCTGTGAGGAACAGTGAAG |
58 |
200 |
| |
R: GCCTTTTATGTGGGTACTCC |
|
|
| 4 |
F: CCCCAGAGGCCATCCCTCCT |
58 |
225 |
| |
R: CCGGGCAGACGGAGGTCATC |
|
|
| 5 |
F: TAAACACAGAGTCTGCAGCC |
58 |
260 |
| |
R: TTCATTATGCACCAAGGGCC |
|
|
| 6 |
F: TGTGTTGACTGCTCATCCTT |
58 |
317 |
| |
R: CATTCAGGGGAACCTGCTCT |
|
|
| 7 |
F: TTCAGGGAGCACTCCATCTT |
55 |
224 |
| |
R: ATCTAGCTGCACAAATGAGG |
|
|
| 8 |
F: CTTGACCTGAGTCTGTTTGG |
58 |
324 |
| |
R: GAAGTCGCCCAAAGATCTCT |
|
|
| 9 |
F: ACTTTTGAACCCACTTTCTC |
58 |
200 |
| |
R: CAATCTAACAGGGATGCCTT |
|
|
| 10 |
F: TCTGGACCTAACCATCACCC |
58 |
206 |
| |
R: CAGGAGCATGATTTAGGACC |
|
|
| 11 |
F: CTCGTGGAAGTATAACCAGT |
58 |
223 |
| |
R: TGGGCAGAAGCTCCACCCGG |
|
|
| 12 |
F: CATTCCAGTGGCCTGGACTCTACTATC |
58 |
318 |
| |
R: GGGGCCCTGAGGGATCACTACTT |
|
|
| 13 |
F: GGGATTAACTCTATCTCCTT |
58 |
249 |
| |
R: TGTGTATAATTCCATCCTGG |
|
|
| 14 |
F: CTGTTCAGTAAACACTTGCT |
58 |
262 |
| |
R: CTCTCCACCAACTGCCACAT |
|
|
| 15 |
F: CACTCTGGTCAAACCTGCCT |
58 |
147 |
| |
R: AGGCTAGGCGCAAACCTAGC |
|
|
| 16 |
F: CAGTTGCAGGTATAACTTTC |
58 |
120 |
| |
R: TAAACAGGTCTGCAATGACT |
|
|
| 17 |
F: GGGAGATCTGCACCTATTTG |
58 |
113 |
| R: TGGTGCATTCCTCCTGTAGT |