Table 1. Primer sequences used for PCR.
Gene | Sense primer | Anti-sense primer | Annealing temperature (° C) | PCR product (bp) |
---|---|---|---|---|
ΔNp63 |
GAAAACAATGCCCAGACTCAATTT |
TCTGCGCGTGGTCTGTGTTAT |
54 |
127 |
K3 |
TCCGTCACAGGCACCAAC |
TGCGTTTGTTGATTTCGTCT |
54 |
175 |
Oct-4 |
CTTGGGCTACACTCAGGC |
CTCAATACTCGTTCGCTTTC |
60 |
238 |
GAPDH | GGAGCCAAAAGGGTCATC | CCAGTGAGTTTCCCGTTC | 54 | 346 |