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. 2010 Jun 10;3:163. doi: 10.1186/1756-0500-3-163

Table 2.

Phenotypic properties of S. saprophyticus strains.

Lipolytic activity Hydrophobicity Collagen adherence
Total Weak High ssp + Total Weak High Total Weak High sdrI +

ATCC 15305 - - - - + - -

7108 + - + + + + + +

clinical isolates (n = 236) 66.5% 84.1% 15.9% 89.2% 75% 42.7% 57.3% 19.9% 25.6% 74.4% 14.9%

animal isolates (n = 15) 46.7% 100% 0% 100% 100% 20% 80% 6.7% 100% 0% 0%

Lipolytic activity was measured on tributyrin agar plates: weak activity: halo > 1 mm, high activity: halo > 3 mm. For measurement of hydrophobicity the hydrophobicity index (HPBI) was calculated from the distribution of the bacteria between an aqueous and an organic solvent phase: weak hydrophobe: HPBI > 10%, high hydrophobe: HPBI > 40%, Adherence to collagen was determined using immobilized collagen type I. The bound bacteria were stained and the optical density was measured: weak adherence: OD > 0.15, high adherence: OD > 0.25.