Table 1.

Fps1 can be produced in a range of microbial cell factories

Production Host	Promoter	Production	Localisation	Reference
S. cerevisiae W301-1A	GAL1	Yes	tm	[38]
S. cerevisiae W301-1A	TPI1	Yes	tm	[38]
S. cerevisiae 954 VW K70	TPI1	Yes	tm	[21] and this study
S. cerevisiae KOY.PK2-1C82	TPI1	Yes	tm	This study
S. cerevisiae TM6*	TPI1	Yes	tm	This study
P. pastoris X-33	AOX1	Yes	pm	[38]
P. pastoris X-33	AOX1	Yes†	nd	[38]
P. pastoris GS115	AOX1	Yes	nd	[38]
P. pastoris KM71	AOX1	Yes	nd	[38]
E. coli JM109	lac	Yes	tm	[38]
E. coli JM109	T7/lac	Yes	tm	[38]

Fps1 was produced in S. cerevisiae, P. pastoris and E. coli as previously described [21,38] and as a part of this study (tm, total membrane; pm, plasma membrane; nd, not determined). Note that ^† indicates that the α-factor signal peptide was used in the expression construct. In all cases prior to this study, total yields were less than 50 μg/L, which was below the threshold value for further study [21,38].