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Effect of IL-11 on STAT3 phosphorylation in human endometrial cancer cell lines. Cells were cultured with IL-11 (1-500 ng/ml) for 15 min. A: ECC-1 cells. B: HEC-1A cells. C: Ishikawa cells. Cell lysates (30 μg protein) were electrophoresed by SDS-PAGE and immunoblotted with anti-p(Tyr705)STAT3 (top panel) or anti-STAT3 (bottom panel) followed by HRP-conjugated rabbit antiserum and visualized by chemiluminescence. cont = control
