Table 1.
Bacteria used in this study.
| Straina | Host plant of isolation | Geographical origin | End Point PCR | Real-Time PCR | ||||
|---|---|---|---|---|---|---|---|---|
| P.savastanoi pv. savastanoi | pathovar- specific primer pairs | pathovar- specific primers/probes | ||||||
| Psv | Psn | Psf | Psv-RT | Psn-RT | Psf-RT | |||
| ITM317, IPVCT-3, LPVM22, LPVM510, LPVM602, ES47b, ES49b, ESB50b, PvBa223 | olive | Southern Italy | + | - | - | + | - | - |
| Legri1b, Legri2b, MC1b, MC33b, MC72b, MC80b, LPVM15, LPVM20 | olive | Central Italy | + | - | - | + | - | - |
| ITMKS1, ITMKL1, ST2 b | olive | Greece | + | - | - | + | - | - |
| 1657-8 c | olive | Spain | + | - | - | + | - | - |
| DAR7635d | olive | Australia | + | - | - | + | - | - |
| P. savastanoi pv. nerii | ||||||||
| ITM519, IPVCT-99, ESC8b, ESC6b, ESC43b, ESB60b, LPVM12, LPVM33, LPVM71, LPVM201, PvBa219 | oleander | Southern Italy | - | + | - | - | + | - |
| ITM601, ES23b, LPVM103 | oleander | Northern Italy | - | + | - | - | + | - |
| NCPPB640 | oleander | Ex-Yugoslavia | - | + | - | - | + | - |
| P. savastanoi pv. fraxini | ||||||||
| NCPPB1006, NCPPB1464 | ash | United Kingdom | - | - | + | - | - | + |
| PD120 | ash | The Netherlands | - | - | + | - | - | + |
| CFBP1838, CFBP2093 | ash | France | - | - | + | - | - | + |
| MCa3b, MCa4b | ash | Italy | - | - | + | - | - | + |
| P. savastanoi pv. phaseolicola 1449Be | Lablab purpureus | Ethiopia | - | - | - | - | - | - |
| P. savastanoi pv. glycinea PG4180f | soybean | New Zealand | - | - | - | - | - | - |
| Unidentified bacteriag | olive, oleander, ash | Central Italy | - | - | - | - | - | - |
a CFBP, Collection Française de Bactéries Phytopathogènes, INRA, Angers, France; IPVCT, Culture collection of Dipartimento di Scienze e Tecnologie Fitosanitarie, Università degli Studi di Catania, Italy (from V. Catara); ITM, Culture collection of Istituto Tossine e Micotossine da Parassiti vegetali, C. N. R., Bari, Italy (from A. Sisto); LPVM, Culture Collection of Laboratorio di Patologia Vegetale Molecolare, Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze; NCPPB, National Collection of Plant Pathogenic Bacteria, York, UK http://www.ncppb.com/; PD, Culture collection of Plant Protection Service, Wageningen, The Netherlands; PVBa, Culture Collection of Dipartimento di Patologia Vegetale, Università degli Studi di Bari, Italy (from A. Sisto).
b from E. Santilli and M. Cerboneschi
c from M. M. Lopez
d from E. J. Cother
e from R. W. Jackson
f from M. S. Ullrich
g bacterial epiphytes naturally occurring P. savastanoi host plants and isolated as described in Methods.