Table 2.
Nucleotide sequences of PCR primers and probes used and developed in this study.
| Primer/Probea | Sequence (5'-3') | Positionb | Product size (bp) | Accession Number |
|---|---|---|---|---|
| PsvF | GGCGATGTTCTCAGCGGATTTG | 24 | 388 | FM253081 |
| PsvR | GATCAAGTGTCCAAGGAAGTGAAGG | FM253082 | ||
| PsvRT-F | CGGATTTGGTTTGCGGGGTA | 38 | 298 | FM253083 |
| PsvRT-R | AATGGGGTGACACTAAAAATTGTGAA | FM253084 | ||
| PsvRT-P | (HEX)CTCGTGCGATCTAAACAGCCGTAGC(BHQ-1)c | 278 | FM253085 | |
| PsnF | ACCCCTCATTGTAACGGATG | 1 | 349 | AM051225 |
| PsnR | TCCCCGGAATTCAACACTTA | AM051226 | ||
| PsnRT-F | GCTCATTCGCTTGTTATCACTTCA | 181 | 169 | AM086621 |
| PsnRT-R | TCCCCGGAATTCAACACTTA | AM051226 | ||
| PsnRT-P | (FAM)TACGCCCGACGCCCGAGCCA(BHQ-1)c | 206 | FM253086 | |
| PsfF | CGCCTGCTGTACTCCTCGG | 1 | 412 | AM055834 |
| PsfR | TCGACCTGTCTAAGGCCC | AM055835 | ||
| PsfRT-F | CAGCTCATCCATTAATAGGGCAAG | 207 | 227 | AM086622 |
| PsfRT-R | GGGCAGTGTCAGGGGATG | FM253088 | ||
| PsfRT-P | (Texas Red)CTTGTACCGAAGCGTGCCGTCTGC(BHQ-2)c | 237 | FM253087 |
a F, forward; R, reverse; RT, RealTime; P, probe.
b Starting nucleotide position of forward primers and TaqMan® probes on target sequences.
c BHQ-1 and BHQ-2 are quencher molecules available from the manufacturer.