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rst2-gfp, rst2-gfp cgs1Δ, rst2-gfp nmt-tor2 cells were grown to mid-exponential phase in minimal medium and shifted to minimal medium lacking nitrogen for 4 hours. In all cases, Rst2-GFP was located in the nucleus, except in the cgs1Δ mutant. rst2-gfp tor2-51 and rst2-gfp tor2-51 cgs1Δ cells were grown to mid-exponential phase in minimal medium at 25°C and then shifted to 32°C to inactivate Tor2. Nuclei were stained with Hoescht.
