Figure 7. Role of oxidative stress in generating the 5 kDa form.

HEK 293 cells were transfected with pEGFP vector alone (vector) or pEGFP-MsrB1-F1 wild-type (WT) for 24 h and then cultured with DMEM containing indicated concentrations of hydrogen peroxide for another 24 h. Protein extracts were resolved on SDS-PAGE and detected by western blot analysis using antibodies specific for MsrB1 (A). MsrB1 band intensities were determined with Image J and MsrB1 short form levels were normalized to those of the EGFP-MsrB1 form (B).