Table 3. QPCR validation of gene expression data.
Microarray24 Hours | Microarray48 Hours | Microarray72 Hours | qPCR72 Hours | ||||
Gene symbol | Median Fold-Change(F/C) | Corrected p Value | Median Fold-Change(F/C) | Corrected p Value | Median Fold-Change(F/C) | Corrected p Value | Average Fold-Change(F/C) |
CCNI | 1/1.68 | 0.26 | 1/2.46 | 0.28 | 1/3.00 | 5.22E-05 | 1/1.54 |
1/2.24 | 0.27 | 1/4.59 | 0.25 | 1/3.31 | 0.0017 | ||
1/2.57 | 0.23 | 1/3.32 | 0.33 | 1/3.44 | 0.0035 | ||
1/2.27 | 0.23 | 1/2.90 | 0.34 | 1/4.21 | 3.63E-04 | ||
WNT5a | 1/3.88 | 0.15 | 1/2.56 | 0.41 | 1/2.31 | 0.017 | |
WNT3 | 1.26 | 0.40 | 1.90 | 0.41 | 2.81 | .023 | |
FZD10 | 1.28 | 0.86 | 1/1.34 | 0.88 | 15.48 | 0.0092 | |
BECN1 | 1.05 | 0.88 | 1.65 | 0.37 | 2.04 | 0.051 | 1.66 |
1.39 | 0.27 | 1.37 | 0.50 | 1.61 | 0.15 | ||
CDC45L | 3.15 | 0.34 | 6.19 | 0.51 | 3.16 | 0.14 | |
DPM1 | 1.10 | 0.79 | 1.51 | 0.19 | 2.40 | 5.00E-04 | 1/1.11 |
1.17 | 0.52 | 1.47 | 0.51 | 2.15 | 0.0024 | ||
SNX1 | 1.69 | 0.21 | 1.22 | 0.39 | 2.65 | 7.07E-04 | 5.62 |
EME1 | 1.03 | 0.27 | 1.03 | 0.65 | 5.29 | 0.0032 | 1.38 |
1.99 | 0.25 | 3.52 | 0.36 | 1.08 | 0.31 | ||
FBXO8 | 1.03 | 0.27 | 2.02 | 0.22 | 3.42 | 0.0072 | 1.96 |
1/1.99 | 0.25 | 2.05 | 0.37 | 2.16 | 0.031 | ||
PIGW | 2.09 | 0.041 | 2.58 | 0.33 | 4.30 | 6.38E-04 | 1.64 |
CDH20 | 1/1.11 | 0.87 | 1/1.28 | 0.56 | 1/8.20 | 0.0018 | 1/8.11 |
1.13 | 0.89 | 1/2.19 | 0.51 | 1/22.25 | 4.52E-05 | ||
ITGA4 | 1.09 | 0.36 | 1/1.18 | 0.51 | 1/1.06 | 0.47 | 1/3.67 |
1/1.87 | 0.080 | 1/1.40 | 0.49 | 1/2.08 | 0.089 | ||
1.16 | 0.47 | 1/1.42 | 0.51 | 1/2.28 | 0.055 | ||
NLGN1 | 1.06 | 0.30 | 1/1.12 | 0.51 | 1.02 | 0.43 | 1/1.46 |
1.08 | 0.27 | 1/1.08 | 0.51 | 1/1.01 | 0.87 | ||
1/5.92 | 0.23 | 1/3.90 | 0.15 | 1/1.25 | 0.45 | ||
1/2.58 | 0.27 | 1/3.08 | 0.51 | 1/2.11 | 0.0046 | ||
1.06 | 0.28 | 1/1.05 | 0.51 | 1/2.99 | 0.011 | ||
CDKN1B (p27) | 1/1.27 | 0.30 | 1/2.97 | 0.49 | 1/2.12 | 0.015 | 2.64 |
1/1.77 | 0.15 | 1/1.72 | 0.22 | 1/2.21 | 9.92E-04 | ||
OCM | 1/1.27 | 0.307 | 1/153.66 | 0.14 | 1/64.33 | 1.81E-04 | 1/13.45 |
QPCR and microarray data for individual genes of interest and genes selected for qPCR validation as described in the text. Multiple microarray values represent different probe sets for the same gene.