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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Epidemiol Biomarkers Prev. 2010 Jun 15;19(7):1766–1774. doi: 10.1158/1055-9965.EPI-10-0027

Figure 1. Total RNA and miRNA detection in the stools of healthy subjects.

Figure 1

(A) The graph shows the variation and amount of total RNA measured with RiboGreen and expression levels of RNU6b measured by TaqMan-PCR. Both total RNA concentrations (B) and RNU6b expression levels (C) showed a significant correlation between independent extractions. (D) Comparison of Ct values detected by qRT-PCR from various miRNAs showed a significant correlation between the direct miRNA analyses (DMA) method and miRNA from Qiagen kit-extracted samples. (E) Significant correlation in miR-16 and -26b expression levels was observed among different healthy individuals (F) Based on previous literature, 3 housekeeping RNA/miRNAs were evaluated for possible use as an internal control. RNU6b showed a high degree of variation and showed no significant correlation with miR-16 and -26b.