Table 2.
Relative fold gene expression in TAA fibroblasts as compared to normal fibroblasts at steady-state and following stimulation with various growth factors at the indicated concentrations; ND = not detected.
| Fold Expression (TAA Fibroblasts vs. Normal Fibroblasts) | |||||
|---|---|---|---|---|---|
| Gene Symbol | Steady-State | ET-1 (1 nM) | PMA (100 nM) | AngII (100 nM) | |
| MMPs/TIMPs | Mmp2 | 6.54 (p = 0.0055) | 4.46 (p = 0.0239) | 3.65 (p = 0.0472) | 5.94 (p = 0.0171) |
| Mmp3 | 0.41 (p = 0.3622) | 0.26 (p = 0.2470) | 0.06 (p = 0.0137) | 0.22 (p = 0.2382) | |
| Mmp9 | 0.09 (p = 0.0488) | 0.04 (p = 0.0012) | 0.21 (p = 0.1109) | 0.09 (p = 0.1828) | |
| Mmp11 | 9.78 (p = 0.0027) | 2.78 (p = 0.2959) | 2.03 (p = 0.1184) | 7.26 (p = 0.0278) | |
| Mmp13 | 1.11 (p = 0.8461) | 0.97 (p = 0.9514) | 0.11 (p = 0.0280) | 0.42 (p = 0.7376) | |
| Mmp14 | 2.01 (p = 0.1014) | 2.13 (p = 0.0008) | 1.78 (p = 0.1450) | 1.87 (p = 0.1579) | |
| Mmp15 | 1.17 (p = 0.8590) | 0.66 (p = 0.3911) | ND | 0.23 (p = 0.0902) | |
| Timp1 | 1.41 (p = 0.2415) | 0.99 (p = 0.9766) | 1.53 (p = 0.1271) | 1.39 (p = 0.4637) | |
| Timp2 | 1.11 (p = 0.5787) | 0.95 (p = 0.8977) | 0.95 (p = 0.8377) | 1.27 (p = 0.3828) | |
| Timp3 | 0.33 (p = 0.1490) | 0.49 (p = 0.3012) | 0.43 (p = 0.0799) | 0.30 (p = 0.0896) | |
| Collagen/Elastic Architecture | Col1a1 | 2.52 (p = 0.0583) | 1.83 (p = 0.0793) | 2.99 (p = 0.0015) | 1.84 (p = 0.2291) |
| Col1a2 | 3.45 (p = 0.0082) | 2.90 (p = 0.0007) | 3.61 (p = 0.0047) | 3.46 (p = 0.0049) | |
| Col3a1 | 4.83 (p = 0.0083) | 3.51 (p = 0.0102) | 4.52 (p = 0.0120) | 4.05 (p = 0.0109) | |
| Col4a1 | 2.82 (p = 0.0039) | 2.36 (p = 0.0284) | 2.22 (p = 0.3715) | 1.94 (p = 0.4345) | |
| Col6a1 | 1.67 (p = 0.2485) | 1.47 (p = 0.4708) | 1.52 (p = 0.5061) | 1.41 (p = 0.4827) | |
| Eln | 2.30 (p = 0.2558) | 2.32 (p = 0.2918) | 0.79 (p = 0.7256) | 2.24 (p = 0.4751) | |
| Ltbp-1 | 0.09 (p = 0.0564) | 0.21 (p = 0.0323) | 0.04 (p = 0.0033) | 0.19 (p = 0.0955) | |
| Ltbp-2 | 1.71 (p = 0.2016) | 1.39 (p = 0.4174) | 1.44 (p = 0.3357) | 1.63 (p = 0.3735) | |
| Fbn1 | 1.83 (p = 0.2042) | 2.13 (p = 0.1269) | 2.33 (p = 0.1862) | 2.55 (p = 0.1643) | |
| Other ECM | Lamb1-1 | 1.66 (p = 0.1437) | 1.61 (p = 0.3753) | 0.99 (p = 0.9830) | 2.02 (p = 0.1812) |
| Lamb2 | 21.96 (p = 0.0378) | 1.36 (p = 0.7058) | 0.05 (p = 0.0194) | 0.56 (p = 0.3979) | |
| Fn1 | 2.23 (p = 0.1582) | 1.96 (p = 0.1730) | 1.56 (p = 0.3862) | 1.83 (p = 0.4078) | |
| Spp1 | 2.43 (p = 0.2790) | 3.15 (p = 0.3129) | 0.17 (p = 0.6975) | 1.15 (p = 0.9537) | |
| Thbs1 | 0.88 (p = 0.5526) | 0.59 (p = 0.0240) | 1.39 (p = 0.2732) | 0.70 (p = 0.3291) | |
| Sparc | 2.10 (p = 0.1208) | 2.25 (p = 0.0624) | 1.40 (p = 0.5110) | 0.90 (p = 0.8262) | |
| Ager | 0.72 (p = 0.4922) | 0.69 (p = 0.5648) | 0.73 (p = 0.5835) | 0.90 (p = 0.8769) | |
| Transcription Factors | Sp1 | 1.68 (p = 0.1245) | 1.21 (p = 0.5112) | 1.22 (p = 0.5883) | 0.97 (p = 0.8670) |
| Sp3 | 0.98 (p = 0.9197) | 1.13 (p = 0.7552) | 1.03 (p = 0.9300) | 0.94 (p = 0.8385) | |
| Jun | 1.49 (p = 0.6169) | 3.71 (p = 0.0106) | 2.63 (p = 0.0460) | 2.28 (p = 0.0174) | |
| Junb | 0.82 (p = 0.7838) | 1.28 (p = 0.7831) | 1.46 (p = 0.4151) | ND | |
| Fos | 2.14 (p = 0.7734) | 1.71 (p = 0.4049) | 0.46 (p = 0.4656) | 0.97 (p = 0.9632) | |
| Fosb | 2.41 (p = 0.3959) | 3.40 (p = 0.2602) | 5.21 (p = 0.1182) | 1.67 (p = 0.6711) | |
| Etv4 | 0.91 (p = 0.7819) | 1.14 (p = 0.7663) | 0.44 (p = 0.2232) | 0.82 (p = 0.6757) | |