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SfiI macrorestriction patterns of E. faecalis PAI transconjugants. Lane 1, lambda DNA ladder standard; lane 2, recipient strain OG1RF; lanes 3–14, transconjugant strains TC1, TC2, TC7, TC4, TC6, TC8, TC9, TC10, TC3, TC12, TC5, and TC11, respectively; lane 15, donor strain V583. PFGE pattern groupings are indicated below the gel. Plasmid pTEF2, 57.7 kb in size and present in 3 of the 12 transconjugants, is indicated by arrows.
