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. 2010 Jun 21;107(27):12216–12221. doi: 10.1073/pnas.1004143107

Fig. 3.

Fig. 3.

PDGF-CC deficiency and blockades inhibited ischemia-induced retinal neovascularization. (A) Real-time PCR showed up-regulated expression of PDGF-CC and PDGFR-α during retinal ischemia. Arbitrary unit was used for gene expression with the d 0 control set to 1. (B) Immunofluorescence staining showed PDGFR-α expression by the neovessels in the retina, with a higher expression level in the neovascular tufts. (C and D) PDGF-CC deficiency reduced retinal neovascularization 5 d after retinal ischemia. (E–G) PDGF-CC nAb intravitreal injection inhibited retinal neovascularization (E and F), leading to greater avascular areas in the retinas (E and G). (H–J) Intravitreal injection of PDGF-CC shRNA decreased retinal neovascularization (H and I), leading to greater avascular areas in the retinas (H and J). (Scale bars: 100 μm in B; 500 μm in C, E, and H.) ** P < 0.01, *** P < 0.001.