Table 2.

Results of primer extension assays without downstream-binding oligonucleotide (Scheme 1B) for the 16 core template sequences CBB², as determined by fitting

Core of template	Conversion* 20 °C, %	k′, 20 °C, h-1 M-1	Conversion* 10 °C, %	k′, 10 °C, h-1 M-1
CAA	80	30	99	20
CAC	81	40	96	20
CAG	69	30	93	20
CAT	99	90	99	100
CCA	99	530	99	490
CCC	99	550	99	340
CCG	97	360	99	320
CCT	99	1,480	99	1,310
CGA	98	150	99	70
CGC	99	310	99	150
CGG	99	240	99	160
CGT	99	660	99	420
CTA	87	70	99	70
CTC	99	150	99	190
CTG	98	90	99	100
CTT	99	280	99	370

Conditions: 3.6 mM OAt-activated monomer, 36 μM aminoprimer, 54 μM template, 200 mM HEPES buffer (400 mM NaCl, 80 mM MgCl₂, pH 8.9).

^*Maximum conversion of primer, as determined by fit.