Table 3.

Kinetic values (sorted by increasing k₁ values) for two-step primer extension as obtained by fitting the model of Eq. 1 to kinetics at different monomer concentrations (1.8, 3.6, 7.2, 18, and 36 mM TMP-OAt) for the 16 different B¹AB² templates

Template	, mM-1 min-1	M*, min-1	k3, min-1
CAG	0.0029	0.0104	0.13
AAG	0.0032	0.0115	0.10
TAG	0.0033	0.0119	0.11
CAC	0.0046	0.0166	0.17
CAA	0.0047	0.0169	0.16
TAA	0.0052	0.0187	0.17
TAC	0.0054	0.0194	0.16
AAA	0.0059	0.0212	0.17
GAG	0.0064	0.0230	0.16
CAT	0.0072	0.0259	0.21
GAA	0.0088	0.0317	0.20
AAC	0.0098	0.0353	0.15
TAT	0.0101	0.0364	0.21
GAC	0.0148	0.0533	0.13
AAT	0.0221	0.0796	0.28
GAT	0.0265	0.0954	0.36

Note, k₂ was found to be smaller than 0.0001 min^-1 in all cases.

^*Calculated for the default concentration of 3.6 mM TMP-OAt.