Table 4.
Rates for two-step primer extension on each of the four different CBC templates either in the presence of a downstream-binding oligomer (third strand) at 20 °C or in its absence at 10 °C, based on fits to data obtained at 1.8, 3.6, 7.2, 18, and 36 mM monomer concentration, using Eqs. 1 and 2; k2 was smaller than 0.0001 min-1 in all cases
| Template | Third strand | °C |
 M*, min-1
|
k3, min-1 |
| CAC | + | 20 | 0.0166 | 0.170 |
| CTC | + | 20 | 0.0508 | 0.220 |
| CCC | + | 20 | 0.1127 | 0.460 |
| CGC | + | 20 | 0.1188 | 0.330 |
| CAC | − | 10 | 0.0014 | 0.035 |
| CTC | − | 10 | 0.0119 | 0.022 |
| CCC | − | 10 | 0.0263 | 0.088 |
| CGC | − | 10 | 0.0126 | 0.140 |
*Calculated for the default concentration of 3.6 mM monomer.