. 2010 Jun 16;107(27):12074–12079. doi: 10.1073/pnas.0914872107

Table 5.

Product distribution for primer extension with a reactivity-adjusted balanced mixture of OAt-esters of the four canonical deoxynucleoside monophosphates (A/C/G/T) for any of the 64 possible core triplets of templates

Template B¹B B²	Product distribution +C/+T/+A/+G	Template B¹B B²	Product distribution +C/+T/+A/+G	Template B¹B B²	Product distribution +C/+T/+A/+G	Template B¹B B²	Product distribution +C/+T/+A/+G
AAA	0/ > 99/0/0	AAC	0/ > 99/0/0	AAG	0/ > 99/0/0	AAT	0/ > 99/0/0
ACA	1/1/5/93	ACC	2/10/4/84	ACG	0/10/6/84	ACT	0/2/2/96
AGA	86/7/5/2	AGC	84/8/6/2	AGG	83/8/8/1	AGT	91/6/3/0
ATA	0/7/93/0	ATC	0/5/94/1	ATG	0/10/90/0	ATT	0/3/97/0
CAA	0/ > 99/0/0	CAC	0/98/0/2	CAG	0/93/7/0	CAT	0/ > 99/0/0
CCA	0/2/0/98	CCC	2/11/8/79	CCG	3/15/10/72	CCT	0/0/3/97
CGA	92/5/2/1	CGC	87/8/4/1	CGG	78/15/7/0	CGT	91/7/2/0
CTA	0/5/95/0	CTC	0/9/90/1	CTG	0/11/89/0	CTT	0/3/97/0
GAA	0/ > 99/0/0	GAC	0/ > 99/0/0	GAG	2/98/0/0	GAT	0/ > 99/0/0
GCA	0/4/3/93	GCC	1/3/1/95	GCG	4/9/6/81	GCT	0/0/2/98
GGA	84/6/9/1	GGC	73/10/15/3	GGG	75/9/16/0	GGT	87/6/5/2
GTA	0/4/96/0	GTC	0/3/97/0	GTG	2/6/91/1	GTT	0/1/99/0
TAA	0/97/3/0	TAC	0/ > 99/0/0	TAG	0/94/6/0	TAT	0/96/4/0
TCA	1/4/2/93	TCC	0/7/7/86	TCG	3/5/5/87	TCT	0/2/2/96
TGA	90/6/4/0	TGC	81/11/7/1	TGG	92/4/3/1	TGT	93/4/2/1
TTA	0/2/98/0	TTC	0/5/93/2	TTG	0/0/ > 99/0	TTT	0/0/ > 99/0

Conditions: Hepes buffer pH 8.9 (200 mM), 3.6 mM dGMP-OAt, 7.2 mM dCMP-OAt, 10.8 mM dAMP-OAt, 22 mM TMP-OAt, 54 μM template and downstream-binding oligonucleotide, 36 μM primer, 20 °C. Conversion of the primers > 99% at the end of the assay in all cases.