Fig. 1.
Cross-priming with cell-associated antigen followed by short-interval booster immunization rapidly generates protective CD8 T-cell immunity. Naïve C57BL/6 (B6) mice received ∼107 irradiated WT or Kb−/−mOva splenocytes (i.v.). (A) Detection by Kb/Ova257 tetramer staining. (B) Kinetics of Ova257-specific CD8 T-cell response (mean frequency ± SEM, n = 3) in PBL. (C) Phenotypic and functional status of Ova257-specific CD8 T cells at day 7 after DC immunization, cross-priming, or virLM-Ova infection (mean ± SEM, n = 3). (D) Kinetics of Ova257-specific CD8 T-cell response (mean frequency ± SEM, n = 3) in PBL with different booster immunizations as indicated. Numbers indicate fold difference at day 54. (E) Bacteria count (mean ± SEM, n = 3) in spleen and liver ∼65 h after a lethal dose of virLM-Ova. LOD, limit of detection. *Statistical analysis was performed using an unpaired, two-tailed t test.