Fig. 5.

A DN ATPase p97/VCP decreases SP activity but not cleavage. (A) Western blotting of 293T cells transfected with different amounts of GFPrem construct in the absence or presence of a DN p97 (p97QQ). Extracts were subjected to Western blotting with antibodies specific for GFP (Top), actin (Middle), or p97 (Bottom). (B) DN p97QQ inhibits Rem function. Two different amounts of GFPrem were transfected with or without the p97QQ expression construct. Luciferase activity is reported as in Fig. 1D.