Fig. 5.
Get4 rescue. (A) Spot plate growth assays of YEp-352 derived rescue plasmids under control of genomic promoters in the BY4741 Get4::KanMX background. Plates consisted of Sc-Ura supplemented with 2 mM CuSO4 and were incubated at 37 °C. The panel is generated from a single plate. “KO” represents transformations with empty YEp-352 vector and “Rescue” represents wild-type Get4. Rescue mutants labels are colored based on no (cyan), moderate (yellow), and strong (red) phenotypes. (B) Get4/5-N with helices shown as cylinders. Sets of mutated residues are shown as sticks with carbons colored based according to phenotype.