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. 2010 Jun 21;107(27):12245–12250. doi: 10.1073/pnas.1007319107

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Fig. 3. — LptD_CSCC is not properly oxidized and is sensitive to both oxidizing and reducing agents. (A) Lawn of ≈5 × 10⁷ cells of NR754 ΔlptD (pET2342lptD_CSCC) in LB top agar was placed on an LB plate with a disk containing DTT. (B) Crude OM preparations from NR754 ΔlptD (pET2342lptD_CCCC), NR754 ΔlptD (pET2342lptD_CSCC), and NR754 ΔlptD (pET2342lptD_CSCS) grown to OD₆₀₀ ≈0.6 were subjected to reducing (+β-ME) and nonreducing (−β-ME) SDS/PAGE followed by LptD immunoblotting. Size (in kilodaltons) of molecular mass markers are shown on the left.