Fig. 1.

NR4A-mediated neuroprotection. (A and B) MACS-sorted neurons were transfected with the indicated oligos for 5 h, received fresh medium for 19 h, and were then treated with rolipram or DMSO for 1 h and subsequently stressed with the indicated concentrations of ionomycin, H₂O₂, or glutamate for 18 h. Cell survival was assayed by adding cell titer reagent and measuring the absorbance at 490 nm. Error bars indicate SD, n = 9; *significant at P < 0.05; **significant at P < 0.01. (C) MACS-sorted neurons infected with eGFP or NR4A2 lentivirus were treated for 18 h with increasing concentrations of ionomycin, glutamate, or H₂O₂. Cell survival was assayed by adding cell titer reagent and measuring the absorbance at 490 nm. Error bars indicate SD, n = 5. (D) MACS-sorted neurons infected with eGFP or the indicated NR4A lentiviruses were treated for 18 h with the indicated concentrations of ionomycin, glutamate, or H₂O₂. Cell survival was assayed by adding cell titer reagent and measuring the absorbance at 490 nm. The values obtained with the different NR4A lentiviruses were normalized to the eGFP lentivirus values. Error bars indicate SD, n = 5.