Table 2.

Second-order rate constants (k_cat/K_M values) for heteropair and mispair synthesis and extension with dMMO2 or a derivative in the template (context I).^a

5′-TAATACGACTCACTATAGGGAGA(Y)
3′-ATTATGCTGAGTGATATCCCTCT(X)GCTAGGTTACGGCAGGATCGC
X	Y	Synthesis (M−1min−1)	Extension (M−1min−1)
MMO2	5SICS	(4.7 ± 0.4) × 107b	(6.7 ± 1.1) × 105b
	MMO2	(1.2 ± 0.1) × 105c	(5.3 ± 0.9) × 103c
	A	(1.0 ± 0.1) × 105c	(4.6 ± 0.2) × 104c
	G	<1.0 × 103c	<1.0 × 103c
	C	<1.0 × 103c	(1.2 ± 0.5) × 106c
	T	<1.0 × 103c	(6.6 ± 0.2) × 105c
5FM	5SICS	(1.4 ± 0.2) × 107	(2.3 ± 0.2) × 106
	5FM	(8.9 ± 0.7) × 105	(2.6 ± 0.8) × 104
	A	(3.0 ± 0.5) × 105	(3.2 ± 0.4) × 105
	G	<1.0 × 103	<1.0 × 103
	C	<1.0 × 103	(2.0 ± 0.4) × 106
	T	(2.4 ± 0.9) × 103	(2.0 ± 0.2) × 106
NaM	5SICS	(3.7 ± 0.4) × 107	(2.7 ± 0.2) × 106
	NaM	(3.4 ± 0.8) × 106	(1.1 ± 0.3) × 104
	A	(7.1 ± 2.9) × 105	(3.9 ± 1.8) × 104
	G	<1.0 × 103	<1.0 × 103
	C	<1.0 × 103	(1.5 ± 0.1) × 106
	T	(5.6± 0.5) × 103	(5.7 ± 0.3) × 105

^aSee Experimental Section for details.

^bRef. ¹⁰.

^cRef. ⁹.