Table 3.

Second-order rate constants (k_cat/K_M values) for heteropair and mispair synthesis and extension with d5SICS in the template (context II).^a

5′-TAATACGACTCACTATAGGGAGC(Y)
3′-ATTATGCTGAGTGATATCCCTCG(X)TCTAGGTTACGGCAGGATCGC
X	Y	Synthesis (M−1min−1)	Extension (M−1min−1)
T	A	(3.3 ± 0.1) × 108	(3.1 ± 0.4) × 108
5SICS	MMO2	(4.8 ± 1.8) × 105	(3.2 ± 0.1) × 105
	5FM	(4.2 ± 0.8) × 106	(1.3 ± 0.6) × 106
	NaM	(4.1 ± 0.1) × 107	(6.8 ± 2.2) × 105
	5SICS	(2.4 ± 0.2) × 105	<1.0 × 103
	A	(5.9 ± 0.8) × 104	(3.6 ± 1.6) × 103
	G	(1.9 ± 0.5) × 105	<1.0 × 103b
	C	<1.0 × 103	<1.0 × 103
	T	(8.5 ± 1.1) × 103	(3.3 ± 0.2) × 105

^aSee Experimental Section for details.