Table 4.

Second-order rate constants (k_cat/K_M values) for heteropair and mispair synthesis and extension with dMMO2 or a derivative in the template (context II).^a

5′-TAATACGACTCACTATAGGGAGC(Y)
3′-ATTATGCTGAGTGATATCCCTCG(X)TCTAGGTTACGGCAGGATCGC
X	Y	Synthesis (M−1min−1)	Extension (M−1min−1)
MMO2	5SICS	(6.6 ± 0.5) × 107	(1.7 ± 0.8) × 106
	MMO2	(1.8 ± 1.2) × 106	<1.0 × 103
	A	(1.7 ± 0.3) × 106	(1.1 ± 0.3) × 104
	G	(7.9 ± 2.1) × 103	<1.0 × 103
	C	(3.0 ± 0.8) × 103	(4.4 ± 1.0) × 105
	T	(5.2 ± 2.7) × 103	(2.0 ± 0.6) × 106
5NaM	5SICS	(5.5 ± 0.7) × 107	(4.1 ± 0.1) × 106
	5NaM	(4.8 ± 1.2) × 106	<1.0 × 103
	A	(1.4 ± 0.4) × 106	(1.4 ± 0.1) × 104
	G	(3.7 ± 0.4) × 103	<1.0 × 103
	C	(6.4 ± 2.6) × 103	(3.0 ± 0.7) × 105
	T	(8.3 ± 1.4) × 103	(8.3 ± 0.6) × 105
5FM	5SICS	(8.0 ± 0.8) × 107	(8.2 ± 0.3) × 106
	5FM	(2.7 ± 0.6) × 106	<1.0 × 103
	A	(3.1 ± 0.1) × 106	(4.4 ± 0.1) × 104
	G	(1.2 ± 0.4) × 103	<1.0 × 103
	C	(7.5 ± 0.6) × 103	(1.5 ± 0.4) × 106
	T	(1.2 ± 0.6) × 104	(4.1 ± 1.0) × 106

^aSee Experimental Section for details.