Figure 4. Reproductive age does not affect the numbers of KiSS1 immunopositive neurons or their response to hormone treatment in the ARC.
Total KiSS1 immunopositive neurons in the ARC are reported as mean ± SEM (F). Cell counts in rats primed with EB or EB + P were combined for both young (EB, N=7; EB + P, N=6) and middle-aged rats (EB, N=5; EB + P, N=7); as described above (young hormone-treated, N=13; middle-aged hormone-treated, N=12). Representative photomicrographs of KiSS1 immunopositive neurons (A–E) in the ARC; A: young control (OVX, oil-primed, N=6), B: middle-aged control (OVX, oil-primed, N=6), C: young hormone, D: middle-aged hormone, E: negative control incubated without primary antibody. There was a significant main effect of hormone treatment [F (1,33)=5, P<0.05]. * = <0.05 hormone vs. control. Scale bar = 100 µm.