Fig. 1.
A. A representative RT-PCR result showing COX-2 mRNA expression levels of HPTFs in response to various doses of IL-1β. HPTFs were cultured on microgrooved silicone surfaces and were treated with five doses of IL-1β (5, 10, 25, 50, and 100 pM) for 4 h. Cells without IL-1β were used as the control. B. IL-1β dose-dependent COX-2 gene expression. IL-1β at 10 pM induced a significantly higher expression level of COX-2 mRNA levels compared to non-treated cells and it appears to be in the linear range of COX-2 gene expression in response to IL-1β treatment.