Table 1.

Primer sequence for RT-PCR

Genes	Primer sequences (forward/reverse)	PCR product size (bp)	Annealing temperature (°C)	Reference
COX-2	5′-TTCAAATGAGATTGTGGGAAAT-3′ 5′-AGATCATCTCTGCCTGAGTATCTT-3′	305	51	(Nakatani et al., 2002)
MMP-1	5′-CACAGCTTTCCTCCACTGCTGCTGC-3′ 5′-GGCATGGTCCACATCTGCTCTTGGC-3′	395	63	(Orlando et al., 1998)
MMP-1 for real-time PCR	5′-AGTGACTGGGAAACCAGATGCTGA-3′ 5′-GCTCTTGGCAAATCTGGCCTGTAA-3′	160	62
GAPDH	5′-TCACCATCTTCCAGGAGCG-3′ 5′-CTGCTTCACCACCTTCTTGA-3′	570	56	(Adam et al., 1999)
GAPDH for real-time PCR	5′-TCGACAGTCAGCCGCATCTTCTTT-3′ 5′-GCCCAATACGACCAAATCCGTTGA-3′	148	62