Subdermal adipose tissue deficiency in the Agpat6−/− mouse. A: Skin cross-sections with hematoxylin/eosin. Arrows indicate the presence of the fat cell layer in wild-type mice but not in Agpat6−/− mice. Magnification, 160×. B: Left, β-galactosidase staining of a paw cross-section from an Agpat6−/− mouse showing AGPAT expression in the subdermal layer. Magnification, 40×. Right, close-up of the boxed region at left. Magnification, 100×. C: Triglyceride (TG) levels (left) and neutral lipid species determined by thin-layer chromatography (right) from skin lipid extracts of chow-fed mice. DAG, diacylglycerol; TAG, triacylglycerol. * P < 0.001.