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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nature. 2010 Mar 21;464(7289):778–782. doi: 10.1038/nature08853

Figure 2. Phagosomes are arrested at the RAB-5(+) stage in sand-1 mutant worms.

Figure 2

(a–j) Compared to wild-type gonads (a,b), sand-1(ok1963) (c,d) and ccz-1(ok2128) (e,f) mutant worms accumulate apoptotic cells within RAB-5(+) (a–f) PtdIns(3)(+) (g–j) phagosomes. Size bar, 10µm. (k–m) Quantitation of RAB-5(+) or RAB-7(+) phagosome (k), phagosomes containing multiple apoptotic cells (l) or PtdIns(3)P(+) phagosomes (m). ***, p<0.001. Data shown represents mean ± s.d., n is shown in Supplementary Table S1. (n–r) Nematodes co-expressing YFP::RAB-5 (o), CFP::RAB-7 (p) and mCherry::SAND-1 (q) (from the same array) were assayed for simultaneous localization to phagosomes containing apoptotic cells. DIC shown in (n), and overlay in (r). (s) Schematic of the genetic pathway for phagosome maturation in the nematode denoting the position of SAND-1 and CCZ-1.