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. 2010 Jun;9(6):926–933. doi: 10.1128/EC.00011-10

Fig. 3.

Fig. 3.

(A) Subcellular localization of EhPNT in wild-type amoebae. Wild-type amoebae were fixed, and an immunofluorescence assay was performed by using anti-EhPNT (red) and preimmune sera. Bar, 10 μm. Arrows and arrowheads indicate representative vacuolar and dot-like structures of EhPNT, respectively. (B) Colocalization of endogenous and exogenous epitope-tagged PNT in E. histolytica. EhPNT-HA-overexpressing amoebae were fixed, and an immunofluorescence assay was performed by using anti-EhPNT (red) and anti-HA (green) antibodies.