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. 2010 Jun;9(6):926–933. doi: 10.1128/EC.00011-10

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Copyright © 2010, American Society for Microbiology

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Fig. 6. — Localization of a series of truncated EhPNT proteins. (A) Schematic representation of HA epitope-tagged or GFP-fused recombinant EhPNT used in the study. Domains, epitope (or GFP), and amino acid numbers are shown. (B) Localization of epitope-tagged or GFP-fused carboxyl-terminally truncated EhPNT recombinant proteins. The transformants expressing EhTS-GFP, EhTS+dIIb-HA, EhTS+dIIb+dIII-HA, EhTS+dIIb+dIII+L-HA, and EhTS+dIIb+dIII+L+dI-HA and EhPNT-HA were subjected to an immunofluorescence assay using anti-HA (or anti-GFP for EhTS-GFP) and anti-EhSec61 α-subunit (or anti-EhDPMS for the EhPNT-HA transformant) antibodies. Bar, 10 μm.