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Microscopy of procyclic form cells exhibiting aberrant morphologies upon TbPRMT6 depletion. (A) Morphological changes evident in PF TbPRMT6 RNAi cells. Cells grown in the presence (+TET) or absence (−TET) of tetracycline for 6 days were fixed to slides, stained with DAPI, and analyzed by microscopy. (B) Images of PF “hydra” cells, characterized by a central body containing nuclei (white arrows) with multiple heads with or without kinetoplasts (white arrowheads).
