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Silver staining of TAP-tagged purified TbPRMT6 complexes. TbPRMT6–Myc-His-TAP was expressed in PF 29-13 cells and purified using standard tandem affinity purification. The eluate was resolved using SDS-PAGE and silver stained. In addition to the major band representing TbPRMT6-MHCBP (TbPRMT6–Myc-His-calmodulin binding protein) (bold arrow), a protein of approximately 45 kDa was observed (thin arrow).
