Fig. 2.

Expression of Pfcrk-3 in asexual blood stages. (A) Northern blot analysis. Total RNA was extracted from synchronized 3D7 parasites (R, rings; T, trophozoites; S, schizonts) and subjected to Northern blot analysis. (Left) pfcrk-3 probe (catalytic domain); (right) pfrhoph2 probe. (B) Western blot analysis. (Left) The immunopurified IgY antibody against VVD (a peptide derived from the Pfcrk-3 catalytic domain) was used to probe extracts from rings, trophozoites, and schizonts. (Right) An extract from unsynchronized asexual parasites (U) was probed with immunopurified IgY directed against PNG, the large insertion within the Pfcrk-3 catalytic domain.